Correction: De Novo Characterization of the Spleen Transcriptome of the Large Yellow Croaker (Pseudosciaena crocea) and Analysis of the Immune Relevant Genes and Pathways Involved in the Antiviral Response
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Copyright: 2014 The PLOS ONE Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Figure 5. Real-time PCR analysis of selected genes. Total RNA was extracted from the spleens of large yellow croakers sampled at 0, 12 and 24 h after poly(I:C) induction. Real-time PCR was used to validate gene expression changes in the pattern recognition receptors (A), signal transducers (B), interferons and interleukin (C), interferon-stimulated genes (D), JAK-STAT pathway (F), and T-cell receptor (TCR) signaling pathway. Increases and decreases in the relative levels of transcripts with respect to the control b-actin gene are shown.P, 0.05, P, 0.01. doi:10.1371/journal.pone.0097471.g005
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De Novo Characterization of the Spleen Transcriptome of the Large Yellow Croaker (Pseudosciaena crocea) and Analysis of the Immune Relevant Genes and Pathways Involved in the Antiviral Response
The large yellow croaker (Pseudosciaena crocea) is an economically important marine fish in China. To understand the molecular basis for antiviral defense in this species, we used Illumia paired-end sequencing to characterize the spleen transcriptome of polyriboinosinic:polyribocytidylic acid [poly(I:C)]-induced large yellow croakers. The library produced 56,355,728 reads and assembled into 108...
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The proximate composition, amino acids and fatty acids composition in the muscle of wild, inshore and offshore cage-cultured large yellow croaker, Pseudosciaena crocea (Richardson, 1846), were determined to identify nutritional differences. Wild fish groups showed highest content of moisture and crude protein, but the lowest lipid content. Offshore cage-cultured fish showed significantly higher...
متن کاملNutrient compositional differentiation in the muscle of wild, inshore and offshore cage-cultured large yellow croaker (Pseudosciaena crocea)
The proximate composition, amino acid and fatty acid composition in the muscle of wild, inshore and offshore cage-cultured large yellow croaker (Pseudosciaena crocea) were determined to identify nutritional differences. Wild fish groups showed highest content of moisture and crude protein, but lowest lipid content. Offshore cage-cultured fish showed significantly higher content of moisture and ...
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The proximate composition, amino acids and fatty acids composition in the muscle of wild, inshore and offshore cage-cultured large yellow croaker, Pseudosciaena crocea (Richardson, 1846), were determined to identify nutritional differences. Wild fish groups showed highest content of moisture and crude protein, but the lowest lipid content. Offshore cage-cultured fish showed significantly higher...
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Vaccination is an effective and safe strategy for combating bacterial diseases in fish, but the mechanisms underlying the early immune response after vaccination remain to be elucidated. In the present study, we used RNA-seq technology to perform transcriptome analysis of spleens from large yellow croaker (Larimichthys crocea) induced by inactivated trivalent bacterial vaccine (Vibrio parahaemo...
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